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Beta-arrestins operate an on/off control switch for focal adhesion kinase activity
Library catalogue. Expand beyond library holdings. Publication Date. This work analyzes the postmetamorphic evolution of the northwestern Alps thanks to a multiscale field geology study integrated by fission track analysis Digital rights.
Loading Rights. Full Text Present-day uplift of the European Alps: Evaluating mechanisms and models of their relative contributions. Tectonics Washington, D. Full Text Repeated spring precipitation shortage alters individual growth patterns in Scots pine forests in the Western Alps. Over the last decades, Scots pine Pinus sylvestris L. Full Text Post-glacial migration of silver fir Abies alba Mill.
Location South-western Alps and adjacent areas, southern France. Methods The work compiles the available palaeoecological data in the south-western Alps Full Text Active deformation at the southwestern Alps—Ligurian basin junction France—Italy boundary : Evidence for recent change from compression to extension in the Argentera massif.
Several hundred earthquakes of small and moderate magnitude occur each year at the southwestern Alps Full Text Rate of fluvial incision in the Central Alps constrained through joint inversion of detrital Be and thermochronometric data. We apply this analysis to the Codera watershed in the Bergell Intrusion, Central European Alps, where rivers have incised into a glacial valley This basin hosts a The goal of this study was to determine the effect of local and large-scale processes on fire frequency during the postglacial period in a subalpine ecosystem Alps, France Full Text The environment of the last hunters-gatherers and first agro-pastoralists in the western Mediterranean region, between the Rhone and the Northern Apennines 7th - 6th millennium cal.Thank you for visiting nature.
We have previously shown that, in normal human airway tissue, localization of the cystic fibrosis transmembrane conductance regulator CFTR can be affected by epithelial maturation, polarity, and differentiation and that CFTR trafficking and apical localization depend on the integrity of the airway epithelium. In this study, we addressed the question of whether the three-dimensional 3-D organization of adult human airway epithelial cells in suspension culture under rotation, leading to spheroid-like structures, could mimic the in vivo phenomenon of differentiation and polarization.
The kinetics of the differentiation, polarity, and formation of the CFTR-ZOezrin complex was analyzed by transmission, scanning, and immunofluorescence microscopy.
Functional activity of the airway surface epithelium was assessed by monitoring the degree of cAMP-stimulated chloride efflux from cultured cells. Our results show that after the initial step of dedifferentiation, characterized by a loss of ciliated cells and disappearance of epithelial subapical CFTR-ezrin-ZO-1 complex, the isolated cells formed 3-D spheroid structures within 24 hours. After 15 days, progressive ciliogenesis was observed and secretory cells could be identified.
In normal human airway epithelial tissue, the cystic fibrosis transmembrane conductance regulator CFTR protein is restricted to the apical membrane domain of well-differentiated epithelial cells such as ciliated cells Denning et al, ; Puchelle et al, We have reported previously that abnormal expression and distribution of the CFTR protein is not only caused by mutations of the CF gene, but may be observed in non-CF airway tissue when the surface epithelium is remodeled and dedifferentiated Brezillon et al,; Dupuit et al, All of these observations suggest that the regulation and transport function of CFTR are dependent on the state of differentiation and polarization of epithelial cell cultures Morris et al, Polarized organization of the organelles, cytoskeleton, and membrane components directly depends on cell-cell adhesion and cell-substratum interactions Ojakian et al, ; Rodriguez-Boulan and Powell, In vitro maintenance of well-differentiated and polarized epithelial cells for long periods is very difficult.
To retain the differentiated and polarized state of the epithelium, several investigators have developed a three-dimensional 3-D culture model in which epithelial sheets derived from human airway cells form free-floating epithelial spheroids in which ciliary activity and ion transports can be preserved for several months Jorissen et al, ; Pedersen et al, ; Ulrich et al, We addressed the question of whether isolated cells from adult human airway surface epithelium, after complete dissociation and subsequent dedifferentiation, could regenerate a well-polarized, junctional, and differentiated surface epithelium in 3-D suspension culture.
We analyzed, in such developed structures, the cAMP-stimulated efflux of chloride from the cells. We show that after an initial dedifferentiation step, isolated epithelial cells form free-floating spheroids and exhibit progressive redifferentiation, polarization, and apical expression of CFTR and associated proteins. After polarization and regeneration of the surface epithelium, the cAMP-stimulated chloride efflux was also restored.
Dissociated airway epithelial cells cultured in suspension under permanent rotation reassembled as 3-D structures during the first 24 hours. Within the first 5 days of culture, spheroids were generally formed of an epithelial monolayer, although in some spheroids, we could observe a two-layer structure Fig.
After 35 days of culture, a columnar epithelium, characterized by the presence of one or two cell layers covered with long microvilli and ciliated cells, could be observed Fig. Histology of the spheroids. A, Phase-contrast microscopy image of spheroid after 5 days of culture showing the characteristic three dimensional 3-D spherical shape.
B, Transmission electron microscopy image of a semi-thin spheroid section observed after 5 days of 3-D culture. The spheroids are generally formed of monolayered epithelial cells with an inner lumen containing cellular debris. C, After 35 days of 3-D culture, a pseudostratified structure characteristic of mature airway epithelium is observed. Using a mouse antibody to Ki67 a nuclear marker of cell proliferation we did not observe any labeling at the different observation time-points 1, 2, 3, 4, 5, 15, 25, and 35 days.Telugu speech to text app
Scanning electron microscopy SEMshowed that, after 5 days of 3-D culture, the spheroids were composed of undifferentiated cells with numerous microvilli Fig. Cilia could not be identified at the same time.
After 15 days, epithelial cells appeared to be more cohesive Fig. After 35 days of culture, confocal microscopy and SEM highlighted the presence of numerous ciliated cells with mature and actively beating cilia all around the 3-D structure Fig. Scanning electron microscopy of the 3-D cell culture.
Romeo P. Graziella V. Albert Vidal A. Dominic W. Adriaan Joh. Adriaen F.P30 freeflight kits
Alan St. Albert BOUE Louis Fr.Focal adhesion kinase FAK regulates key biological processes downstream of G protein-coupled receptors GPCRs in normal and cancer cells, but the modes of kinase activation by these receptors remain unclear. This is a preview of subscription content, log in to check access.
Rent this article via DeepDyve. BMC Genomics Nikolopoulos SN, Giancotti FG Netrin-integrin signaling in epithelial morphogenesis, axon guidance and vascular patterning.
Cell Cycle 4 3 :e— Rozengurt E Mitogenic signaling pathways induced by G protein-coupled receptors.Samsung j6 odin fail
J Cell Physiol 3 — Prog Biophys Mol Biol 71 3—4 — Biochim Biophys Acta 2—3 — J Cell Sci Pt 7 — Cancer Metastasis Rev 28 1—2 — J Biol Chem 50 — FAK Oncogene 11 10 — Nature — Circ Res 92 3 — Clin Cancer Res 22 15 — Franchini KG Focal adhesion kinase—the basis of local hypertrophic signaling domains. J Mol Cell Cardiol 52 2 — Nat Rev Cancer 14 9 — Curr Protein Pept Sci 12 1 — Mol Cancer Ther 17 4 — Biomed Res Int Nat Rev Mol Cell Biol 11 11 — Prog Biophys Mol Biol 1 — Cancers Basel.
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